Nueva prueba diagnóstica desarrollada en Australia detectó el primer caso de influenza porcina en ese país

Cuando el primer caso de influenza porcina H1N1 fue reportado en México en abril, los investigadores de la universidad de Queensland se dieron a la tarea de desarrollar una prueba para diagnosticar el virus.
En menos de dos semanas el Dr. David Whiley y su equipo de cinco científicos fueron capaces de aportar al departamento de patología con dos nuevos métodos de detección, uno de los cuales fue empleado en el diagnóstico del primer caso australiano de influenza porcina
Desde entonces la prueba ha sido implementada en el Townsville Hospital y el Royal Darwin Hospital, y fue también empleada para detectar el primer caso de influenza porcina en el Territorio del Norte.

http://www.eurekalert.org/pub_releases/2009-09/ra-tda090309.php

”We have a highly skilled team of scientists, with extensive experience and knowledge in molecular diagnostics,” Dr Whiley said.
“When news of swine flu broke we realised we had very little time – possibly days – before the first cases arrived in Australia.
“We therefore temporarily suspended all other research projects so that our team could focus on developing the swine flu assays.
“Ours were certainly the first assays implemented for routine swine flu screening in Australia.”
The assays, which are used to test respiratory specimens such as nose swabs, target two key parts of the virus: the haemagglutinin and neuraminidase genes.
They detect the virus by using the polymerase chain reaction (PCR) technique to amplify these parts of the virus’s genetic material.
“Once the genetic material begins amplifying, positive fluorescent signal is produced via the use of a fluorescent probe,” Dr Whiley said.
“This is all monitored using computer software.”
This is not the first time the team has been tasked with developing a diagnostic test at short notice.
Since 2000, the laboratory has had an ongoing partnership with Pathology Queensland in an assay development program.
“Our laboratory develops new rapid molecular assays for detecting microbial pathogens, and once validated the assays are then transferred to the Molecular Diagnostic Unit of Pathology Queensland for routine use,” Dr Whiley said.
“To date, our laboratory has developed and transferred to Pathology Queensland more than 70 rapid diagnostic tests targeting a range of infectious diseases.
“Therefore it was up to us to make sure Pathology Queensland was equipped with the right methods to detect swine flu.”
Details of the assays were published in the July issue of the Journal of Clinical Virology.